Thứ sáu, 25/04/2025 | 15:34 GMT+7
SUMMARY This study aimed to develop a suitable medium for slow growth conservation oftobacco(Nicotiana tabaccum L)germplasm.The result showed that among different combinations of osmotic agents (sucrose and mannitol),supplemented 20 gL -1 of D-mannitol suited best for slow growth conservation,used for increasing the subculture period.This medium reduced the growth rate and the length of the plants, increased time in a periodic subcultures(45 - 64% compared to the control), reaching 248 - 280 daysand ensured the quality of plants at 200C conditions. It is necessary to continue to evaluate this promising medium to preserve more tobacco genetic resources in the near future. Keywords: slow growth conservation, D-Mannitol, medium, tobacco germplasm Nicotiana tabaccum L. |
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03/04/2025